细粒棘球绦虫原头蚴microRNA表达谱分析

doi:10.11843/j.issn.0366-6964.2018.11.020

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (11): 2477-2485.doi: 10.11843/j.issn.0366-6964.2018.11.020

细粒棘球绦虫原头蚴microRNA表达谱分析

王正荣¹, 薄新文^1*, 张艳艳¹, 马勋², 卢平萍^1,2, 徐梦飞^1,2, 孟季蒙¹

1. 新疆农垦科学院畜牧兽医研究所, 省部共建绵羊遗传改良与健康养殖国家重点实验室, 石河子 832000;
2. 石河子大学动物科技学院, 石河子 832000

收稿日期:2018-03-26 出版日期:2018-11-23 发布日期:2018-11-23
通讯作者: 薄新文,研究员,E-mail:851661263@qq.com
作者简介:王正荣(1984-),男,甘肃定西人,助理研究员,硕士,主要从事家畜寄生虫学与寄生虫病研究,Tel:0993-6683358,E-mail:wzrtiger@sina.com
基金资助:
国家重点基础研究发展计划（973计划）（2015CB150300）；国家自然科学基金（31360608；31860701）；新疆生产建设兵团国际科技合作（2017BC003）

microRNA Profile Analyses of the Protoscoleces in Echinococcus granulosus

WANG Zheng-rong¹, BO Xin-wen^1*, ZHANG Yan-yan¹, MA Xun², LU Ping-ping^1,2, XU Meng-fei^1,2, MENG Ji-meng¹

1. State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Institute of Animal Husbandry and Veterinary, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China;
2. College of Animal Science and Technology, Shihezi University, Shihezi 832000, China

Received:2018-03-26 Online:2018-11-23 Published:2018-11-23

摘要/Abstract

摘要：

旨在解析miRNA在细粒棘球绦虫原头蚴中的表达谱特性，为进一步揭示miRNA在细粒棘球绦虫原头蚴发育中的作用及调控机制提供理论依据。从患病绵羊肝无菌采集细粒棘球绦虫原头蚴，提取总RNA，构建原头蚴miRNA文库，利用RNA sequencing技术进行测序分析。结果表明，本研究从细粒棘球绦虫原头蚴中分离得到109个已知的miRNA分子，同时得到189个新的miRNA发夹前体分子。靶基因的预测结果显示，已知miRNA共有132个靶基因，而新发现的miRNA共有3 152个靶基因。进一步的研究发现这些miRNA分子广泛参与虫体生长发育相关的关键信号通路，诸如wnt、cAMP、Hedgehog、NF-κB、Notch以及胆酸盐等信号通路，研究又进一步采用实时荧光定量PCR以及原位杂交技术对虫体经典wnt信号通路相关的miRNA分子以及其靶标基因进行了差异表达以及组织定位研究。结果提示，HG328413.1_36258和β-catenin、HG328414.1_36364*和dis以及HG328399.1_25846和axin可能存在潜在的调控关系。综上所述，本研究在细粒棘球绦虫原头蚴中发现了大量的新的miRNA分子，丰富了原头蚴miRNA的数据，为进一步解析miRNA在细粒棘球绦虫原头蚴发育中的作用奠定了基础。

Abstract:

The purpose of this study was to analyze the expression profile characteristics of miRNA of the protoscoleces in Echinococcus granulosus and to provide theoretical evidence for further revealing the role of miRNA in the development of the protoscoleces. The protoscoleces were collected from the infected sheep liver under sterile condition, and the total RNA was extracted. Then the miRNA Library of the protoscoleces was constructed and sequenced by RNA sequencing technology. The results showed that 109 known miRNA molecules were isolated from the protoscoleces, and 189 novel miRNA hairpin precursors were detected in the PSC of the E. granulosus. The target gene prediction results showed that these known miRNAs had 132 target genes, and the novel miRNAs had 3 152 target genes. A further study found that these miRNA molecules are widely involved in growth and development of key signaling pathways, such as Wnt, cAMP, Hedgehog, NF-κB, Notch and bile salt pathway. miRNA molecules were further researched by real-time quantitative PCR and in situ hybridization. The results suggested that there may be potential regulatory relationships between HG328413.1_36258 and β-catenin, HG328414.1_36364* and dis, as well as HG328399.1_25846 and axin. To sum up, a large number of new miRNA molecules were found in the E. granulosus in this study, which enriched the data of miRNA of the protoscoleces, and laid a foundation for further analysis of the role of miRNA in the development of protoscoleces in E. granulosus.

中图分类号:

S852.734

王正荣, 薄新文, 张艳艳, 马勋, 卢平萍, 徐梦飞, 孟季蒙. 细粒棘球绦虫原头蚴microRNA表达谱分析[J]. 畜牧兽医学报, 2018, 49(11): 2477-2485.

WANG Zheng-rong, BO Xin-wen, ZHANG Yan-yan, MA Xun, LU Ping-ping, XU Meng-fei, MENG Ji-meng. microRNA Profile Analyses of the Protoscoleces in Echinococcus granulosus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(11): 2477-2485.

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细粒棘球绦虫原头蚴microRNA表达谱分析

microRNA Profile Analyses of the Protoscoleces in Echinococcus granulosus

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